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Chapter OP-9

PMI-based positive selection system for genetic transformation of bread wheat T. aestivum

Dr. Neetu Chaudhary

Abstract

E. coli PMI gene can be used for positive selection-based genetic transformation of bread wheat. PMI gene expressed in wheat cells confers ability to utilize mannose as carbohydrate source.

Keywords: PMI gene; Wheat transformation; Positive selection; Selectable marker genes; Transgenic crops

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